![]() ![]() The key factors in ascidian mortality varied between the two species, but for both species, the treatment and salinity were dominant predictors for survival. We used survival analysis methods to locate predictors of ascidian mortality Environmental conditions at ports with high mortality were used to identify similar major ports on a global scale as potential abiotic barriers. We tested the effect of each treatment and the varying environmental conditions on the survival of two invasive ascidians (Chordata, Ascidiacea). Here, we conducted a controlled simulation of key abiotic factors that determine marine bioinvasion success: temperature, salinity, and food availability along selected routes, under two treatments: ULCV and intermediate-size vessels. Understanding the risk of NIS introduction by this emerging shipping category is needed for devising strategies for sustainable shipping. A dominant trend in the shipping industry is the operation of Ultra Large Container Vessels (ULCV), which are over 395 m long and sail mostly on the East-Asia – northern-Europe route. NIS distributions are often a consequence of frequent trade activities that are affected by economic trends. The shipping industry is considered the main vector of introduction of marine non-indigenous species (NIS). ![]()
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